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MetaFluor User’s Guide
Acquire Stream (Run Experiment Menu)
Acquires images from one or two wavelengths as rapidly as possible into
computer RAM memory.
Use this command to perform high-speed acquisition of images. The number of wavelengths
and images to be acquired is defined by the Stream Setup command. If you have a Physik
piezo focuser and the appropriate acquisition hardware (see Hardware, below), you can also
use the Stream commands to perform Z-streaming. After the images have been acquired into
RAM, MetaFluor will transfer the image data to disk.
Before using this command, you must use the Stream Setup command to specify the
wavelength, number of frames to acquire, the camera state, the shutter mode, and the clear
mode. You must also use the Configure Acquisition command to define other acquisition
settings.
Note: This command is unavailable in the MetaFluor Offline system.
Hardware
The fast acquisition mode used by the Acquire Stream command is currently supported by the
following video hardware:
All Photometrics cameras,
All Princeton Instruments cameras,
All video cameras connected to the Flashbus video acquisition board, and
All cameras (video and digital) connected to the MuTech video acquisition board.
Two-wavelength streaming is currently supported only by the Princeton Instruments PentaMax
or MicroMAX camera, or by the MuTech board in combination with a monochrome camera-and then only if you are using a Sutter Lambda 10, Lambda 10-2, or DG4 filter wheel, or a
Polychrome monochromator as your illumination device.
WARNING: If you attempt to use a streaming exposure time that is shorter than the fastest
readout time your camera can handle, your actual exposure time will be limited by the readout
time. If this occurs, MetaFluor will display a warning message.
See Also:
Stream Setup
Experiment Control Panel - Digital Camera
Configure Acquisition
MetaFluor
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Version 7.0