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MetaFluor User’s Guide
Event Marks (Run Experiment Menu)
Marks the time at which a significant event occurred.
Use this command when you want to record a significant event during the experiment in a log
file. For example, you may want to record the time that you added a receptor agonist to your
perfusion medium. You can use the Event Marks command to annotate your data log with the
desired text. Each text line will have the current graph time associated with it. If you are saving
images to play back later, MetaFluor will also record the event in the .inf file associated with
the images so that it can display the event text at the appropriate time as you play back the
images.
The Event Marks dialog box can be expanded and condensed using the More >> and Less <<
command buttons. When the dialog box is in its expanded form, you can compose text for the
event mark in the Event Text box. Additional options will be available for configuring the use of
the experimental timer. Use the Mark New Event Now command button to log newly created
events immediately. Events can be added to the Stored Events List using the Add New Event
to List command button. After events have been added to the Stored Events List, you can log
stored events from the dialog box in its condensed form, either by double-clicking the entry in
the Stored Events List, by choosing the F5: Mark command button, or by pressing the [F5]
function key on your keyboard.
Adding predetermined events to the Stored Events List before starting is usually the best
approach to use for a new experiment with fast acquisition intervals. You can then log the
event marks as needed during the experiment. If you choose this approach, you will be able to
condense the dialog box using Less << after you have added your events to the list; all the
commands you need to log stored events will still be available in the condensed dialog box.
Note: If you find that logging events is a little tricky, it may be that the Timelapse interval you
selected using Timed Acquisition (Experiment Control Panel) is too short. A very short interval,
combined with a large number of Frames to Average (Experiment Control Panel), may not
allow MetaFluor enough time to handle image acquisition and other tasks, such as logging
events.
See Also:
Experiment Control Panel:
For Video Camera with External Monitor
For Video Camera with Computer's Monitor
For Digital Camera
For Playback
MetaFluor
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Version 7.0